BACTERIOLOGY COURSE OUTLINE
PROGRAMME: SCIENCE LABORATORY TECHNOLOGY MICROBIOLOGY
COURSE TITLE: BACTERIOLOGY
COURSE CODE: STM311 CONTACT
HOUR: 5 Hours
GOAL: This course is designed to provide the student
with a good knowledge of bacteria, its structure, taxonomy, nature and economic
importance
UNIT: 3.0
COURSE SPECIFICATION: 1.0 Understand the Bacterial
Structure
Theory Content: Practical
Content
General Objectives:
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Week
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Specific
Learning Objectives
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Teachers
Activities
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Learning Resources
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Specific
Learning Objectives
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Teachers
Activities
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Learning
Resources
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1-2
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ANATOMY
AND MORPHOLOGY Lecture
OF
BACTERIA
1.1
Describe with examples the
bacterial
forms
Microscope
principal
Eubacteria
as revealed by light answer technique
microscopy
e.g. cocci, S.cilli,
cocco-bacilli
etc.
1.2
Describe
the
chemical
composition
of
the
bacterial
cell wall
1.3
Describe bacterial cytoplasmic
ultra-structures
1.4
Explain the form and structures
of
bacterial flagella, pilli and
capsules
1.5
Explain Protoplast,
Sphaeroplasts,
L-forms
And
Mycoplasma
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Lecture
Assignment,
Question
And
answer technique
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Microscope
Incubators
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Prepare
slides, observe Supervise students
and
bacteria
forms under the view and draw
microscope
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Supervise
students to prepare stain, view and draw bacterial forms
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Slides
of bacterial
culture
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COURSE SPECIFICATION: 2.0 Understand the Classification
of Bacterial
Theory Content: Practical
Content
General Objectives:
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Week
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Specific
Learning Objectives
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Teachers
Activities
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Learning Resources
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Specific
Learning Objectives
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Teachers
Activities
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Learning
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3-4
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CLASSIFICATION
OF BACTERIA
2.1
Explain bacterial Class, Order,
Family,
Genus, Species and Strain
2.2
Explain the anatomical and Questions
physiological
properties used as answer techniques
criteria
in the development of the
determinative
key in bacteriology
2.3
Draw
a chart of bacterial
taxonomy
based on 2.2 above
2.4
Explain
bacterial taxonomy
based
on DNA homology
2.5-Explain
numerical taxonomy-
2.6
Explain biochemical taxonomy
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Lecture
Assignment,
Question
And
answer techniques
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Blackboard
Projectors
Taxonomic
Charts
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COURSE SPECIFICATION: 3.0 Understand the Identification
of Bacterial
Theory Content: Practical
Content
General Objectives:
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Week
|
Specific
Learning Objectives
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Teachers
Activities
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Learning Resources
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Specific
Learning Objectives
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Teachers
Activities
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Learning
Resources
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5-6
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IDENTIFICATION
OF BACTERIA
3.1
Explain the following staining
terminologies;
Mordant,
Accentuators,
Vital Staining
3.2
Differentiate between acid, basic positive and Gram-negative bacteria Test
tubes and neutral staining.
3.3
Explain the formulation of stains
important
in the identification of bacteria
3.4
Explain the principles of the reactions of the stains in 3.3 above.
Practical
3.5
List and explain the principles of
Various
biochemical methods used in bacterial identification.
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Lecture
Assignment,
Question
And
answer technique
|
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Carry
out Biochemical
Tests
e.g. sugar fermentation reactions, methyl red, MRVP etc.
Students
to prepare different reagents used for 3.1
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Supervise
Students
To
carry out
Biochemical
of different Gram-positive and Gram-negative bacteria e.g. sugar methyl red,
MRVP etc.
Grade
practical Notebooks
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Glass
wares
Incubators
Slides
Test
tubes
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COURSE SPECIFICATION: 4.0 Understand Bacterial Cultures
and Growth Characterization
Theory Content: Practical
Content
General Objectives:
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Week
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Specific
Learning Objectives
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Teachers
Activities
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Learning Resources
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Specific
Learning Objectives
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Teachers
Activities
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Learning
Resources
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7-9
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BACTERIAL
CULTURES
AND
GROWTH CHARACTERISTICS
4.1
Explain factors which influence
bacterial
growth under laboratory
conditions
e.g. pH, temperature,
Moisture,
nutrient requirements etc.
4.2
Explain types of culture media, e.g. Enriched media, Differential media etc.
4.3.
Explain the preparation and storage of various bacteriological media.
4.4
Identify examples of various bacteriological media in 4.3 above
4.5
Describe various methods of anaerobic incubation e.g.
Anaerobic
jar.
4.6
Describe medium preparation Use of and the cultivation of bacteria e.g. Microaerophilic
Neisseria spp.
4.7
Describe the various methods for the separation of mixed cultures.
4.8
Explain the quality control
methods
of various prepared media of Describe methods
maintenance
and storage of stock culture
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Lecture
Assignment,
Question
And
answer technique
Draw
and explain the Bacterial Growth Curve.
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Prepare
various media for
isolation
and identification
of
specific bacteria
Mannitol
Salt
agar
for
Staphylococcus
aureus etc.
Inoculate
bacteria on media in plate, tube and bottle.
Cultivate
Anaerobic
bacteria using anaerobic jar.
Isolate
And
identify the organism in 4.6 above.
Prepare
media for special purposes e.g.
Transport
media,
media
for
growth
of
Spirochaetes, Leptospira and Mycoplasma.
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Prepare
and
Stock,
store
Culture.
Carry
out the
inoculation
of
Different
bacterial media tube plates and bottle.
Prepare
different
microbiological
Media
for different uses.
Isolate
common
Bacteria.
Carry
tests
to identify
common
Bacteria.
Carry
out
cultivation
of
anaerobic bacteria.
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Glass
ware
Autoclave
Hot
Air Oven
Water
bath
pH
Meter
Weighing
balances
Hot
plates
with
Magnetic
stirrers
Thermometer
Refrigerator
Inoculating
loops
Anaerobic
jar
UV-lamps
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COURSE SPECIFICATION: 3.0 Understand the Identification
of Bacterial
Theory Content: Practical
Content
General Objectives:
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Week
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Specific
Learning Objectives
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Teachers
Activities
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Learning Resources
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Specific
Learning Objectives
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Teachers
Activities
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Learning
Resources
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10-12
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TAXONOMIC
GROUPS
OF
BACTERIA
5.1
Identify the
Bacterial
taxonomicorders.
5.2
List Families and Genera of the Orders.
5.3
Explain the morphological, cultural Biochemical and antigenic Properties as well
as economic Importance of groups the following of bacteria:
a)Gramnegative
aerobicrodsandcolic-
Pseudomonadaceae,
Acetobacter
iaceae,
Rhizobiaceae,
Brucellaceae.
b)
Gram negative;
facultatively
anaerobic rods,
Enterobacteriaceae,
Vibrionaceae.
c)
Gram negative,anaerobic rods-
Bacteriodaceae.
d)
Gram negative heterotrophicaerob
iccocciandcoccibacilli,
Neisseriaceae.
e)
Gram negative, heterotrophic
anaerobiccocci-Veillonellacea.
f)
Gram negative,
chemolithotrophicrods
and cocci-
Nitrobacteriacea,
Siderocapsaseae.
g)
Achaebacteriae.g.Gramnegative
methane-producingrods
and
cocci.
h)
Gram
positiveaerobic/facultativelyanae
robic
cocciStreptococcaceae.
i)
Gram positiveanaerobiccocci
Peptococcacceae.
j)
Gram positive endosporeforming
rods
– Bacillaceae
k)
Gram positive as porogenous rods - Lactobacillaceae.
l)
Actinomyces and related organisms
Streptomycetaceae,
Planaceae,
Actinomycetaceae,
Mycobacteriaceae,
Propioni-
Bacteriaceae
m)
Spirochaetes
n)
Genera of uncertain affiliation
(differentiate
as far as possible)
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Lecture
Assignment,
Question
And
answer technique
|
Taxonomic
Charts, or Tables
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Identify
examples of the organisms in 5.3 above.
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Practical
identification.
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Autoclave
Incubators
Microscopes
Glass
ware
Anaerobic
jar
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COURSE SPECIFICATION: 6.0 Understand Sterilization and Disinfectant
Methods
Theory Content: Practical
Content
General Objectives:
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Week
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Specific
Learning Objectives
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Teachers
Activities
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Learning Resources
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Specific
Learning Objectives
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Teachers
Activities
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Learning
Resources
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13-14
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STERILIZATION
AND DISINFECTION METHODS
6.1
Explain sterilization disinfection by physical methods.
6.2
Explain sterilization and disinfections by
Chemical
methods.
6.3
Sterilize and disinfect various
objects
by physical and chemical
Methods.
6.4
Explain quality control practices involved in 6.1-6.3 above.
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Lecture
Assignment,
Question
And
answer technique
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Practical
Sterilization and disinfection
Carry
out sterilization of different materials by chemical and physical methods.
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Laboratory
demonstrations activities.
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Autoclave
Refrigerator
Bunsen
Burner
Hot
air Oven
Glass
ware
Inoculating
loops
Knives,
Scalpels
UVlamps
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